Analysis of indole derivatives in methanolic extracts from mycelium of Agaricus bisporus cultured in vitro on liquid Oddoux medium

Bożena Muszyńska; Katarzyna Sułkowska-Ziaja; Patrycja Hałaszczuk; Remigiusz Krężałek; Maciej Łojewski

doi:10.2478/fobio-2014-0002

Authors

Bożena Muszyńska Jagiellonian University Medical College, Faculty of Pharmacy, Department of Pharmaceutical Botany
Katarzyna Sułkowska-Ziaja Jagiellonian University Medical College, Faculty of Pharmacy, Department of Pharmaceutical Botany
Patrycja Hałaszczuk Jagiellonian University Medical College, Faculty of Pharmacy, Department of Pharmaceutical Botany
Remigiusz Krężałek Jagiellonian University Medical College, Faculty of Pharmacy, Department of Pharmaceutical Botany
Maciej Łojewski Jagiellonian University Medical College, Faculty of Pharmacy, Department of Pharmaceutical Botany

DOI:

https://doi.org/10.2478/fobio-2014-0002

Keywords:

Agaricus bisporus, in vitro culture, L-tryptophan, serotonin

Abstract

Methanolic extracts obtained from biomass of Agaricus bisporus (J.E. Lange) Imbach cultured in vitro were analyzed for qualitative and quantitative composition of non-hallucinogenic indole compounds in order to compare their amount with fruiting bodies of these species. Extracts demonstrated to contain six indole compounds. Contents of individual compounds ranged from 0.01 to 21.33 mg/100 g d.w. in biomass from in vitro cultures. The quantitatively dominating compounds included: 5-hydroxytryptophan (12.50 mg/100 g d.w.), L-tryptophan (14.00 mg/100 g d.w.) and serotonin (7.00 mg/100 g d.w.). Total content of the remaining indole compounds under analysis in the study was 55.32 mg/100 g d.w.

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