The role of the cytokinin biosynthesis pathways in the rate of tobacco leaf senescence

Paulina Węzigowska; Magdalena Trojak; Ernest Skowron

doi:10.18778/1730-2366.18.08

Authors

Paulina Węzigowska Jan Kochanowski University of Kielce, Biotechnology, Poland
Magdalena Trojak Jan Kochanowski University of Kielce, Department of Environmental Biology, Poland https://orcid.org/0000-0002-5771-2047
Ernest Skowron Jan Kochanowski University of Kielce, Department of Environmental Biology, Poland https://orcid.org/0000-0001-5330-9122

DOI:

https://doi.org/10.18778/1730-2366.18.08

Keywords:

dark-induced leaf senescence, cytokinin biosynthesis, stay-green phenotype

Abstract

The regulation of leaf senescence depends on endogenous and exogenous factors, among them phytohormones like cytokinins (CKs). CKs are key players regulating the senescing process, as their endogenous concentration is linked to the onset and rate of senescence progression. Thus, this study aimed to identify the relationship between the activity of endogenous CKs biosynthesis pathways - the cytosolic mevalonate (MVA) and the plastid methyl-erythritol phosphate (MEP) and the rate of leaf senescence. To this end, three distinct tobacco (Nicotiana tabacum L.) cultivars – Xanthi, Golden Virginia and Monte Calme Yellow were analysed. The study involved treatment with exogenous CK – benzyladenine – and two different CK synthesis inhibitors: lovastatin and clomazone. The progression of senescence was induced by light deprivation and monitored with chlorophyll level (SPAD), photosynthetic activity (PAM) and changes in the Rubisco protein profile (SDS-PAGE). Analyses showed that the Xanthi cultivar was characterized by delayed onset of senescence and stay-green phenotype, while Golden Virginia, and particularly Monte Calme Yellow showed rapid leaf senescence. The studies provided valuable information regarding the role of MEP and MVA pathway of CK synthesis in the regulation of tobacco leaf senescence.

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